SREBP1

From FactorBook

SREBP1
PDB ID : 1AM9
PDB	1AM9
HGNC(alias)	SREBF1 (bHLHd1,SREBP1,SREBP-1c)
Gene Card	SREBF1
Entrez	6720
RefSeq	NM_004176
UniProt	P36956
UCSC	Browser view
Wikipedia	SREBF1
Protein Family	HLH, helix-loop-helix DNA-binding domain
Type	Pol II TF
Ensembl Exp.	Human

Function

SREBP1 (sterol regulatory element binding transcription factor 1, also known as SREBF1) is a transcription factor that binds the sterol regulatory element-1 (SRE1) sequence 5'-ATCACCCCAC-3' found in the flanking region of the LDRL and some genes involved in sterol biosynthesis and lipid homeostasis. It also binds an E-box motif (5'-ATCACGTGA-3'). SREBP1 is synthesized as a precursor attached to the nuclear membrane and ER. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. SREBP1 is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. It is located within the Smith-Magenis syndrome region on chromosome 17.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.

Average Profiles of Nucleosomes around the Summit of ChIP-seq Peaks

No Available Data

Motifs Enriched in the Top 500 ChIP-seq Peaks

The sequences of the top 500 TF ChIP-seq peaks were used to identify enriched motifs de novo, using the MEME-ChIP suite of tools. Five motifs are reported (M1 to M5), with motif name, sequence logo, and number of peaks out of the top 500 peaks that contain a site for the motif. The motifs are then used to scan the entire set of ChIP-seq peaks and the two flanking (control) regions using the FIMO tool, and two quantities are reported for bins of peaks sorted by their ChIP-seq q-values: percentage of the peaks that contain a site for the motif, and the distribution of the distances of the motif site to the summit of the peak.

Binding of Other Transcription Factors or Histone Marks at the SREBP1 Peaks