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interferon regulatory factor 3

PDB1J2F 2O61 2O6G 1T2K 1ZOQ 2PI0 3A77 1QWT
Gene CardIRF3
UCSCBrowser view
Protein FamilyWinged helix DNA-binding domain
TypePol II TF
Ensembl Exp.n/a


IRF3 (interferon regulatory factor 3) belongs to the IRF family of transcription factors, characterized by a unique tryptophan pentad repeat DNA-binding domain. IRFs play an important role in regulating interferons in response to viral infection, and the regulation of interferon-inducible genes as well. IRF3 resides in the cytoplasm in an inactive state. dsRNA from a viral infection leads to phosphorylation of IRF3 on its C-terminal serine/threonine cluster. This induces a conformational change, dimerization, and formation of the dsRNA-activated factor 1 (DRAF1) complex with CREB binding protein (CREBBP). This complex translocates to the nucleus and activates the transcription of genes under the control of ISRE, such as interferons alpha and beta. DRAF1 binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

HeLa-S3 1

Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.

Average Profiles of Nucleosomes around the Summit of ChIP-seq Peaks

No Available Data

Motifs Enriched in the Top 500 ChIP-seq Peaks

The sequences of the top 500 TF ChIP-seq peaks were used to identify enriched motifs de novo, using the MEME-ChIP suite of tools. Five motifs are reported (M1 to M5), with motif name, sequence logo, and number of peaks out of the top 500 peaks that contain a site for the motif. The motifs are then used to scan the entire set of ChIP-seq peaks and the two flanking (control) regions using the FIMO tool, and two quantities are reported for bins of peaks sorted by their ChIP-seq q-values: percentage of the peaks that contain a site for the motif, and the distribution of the distances of the motif site to the summit of the peak.

Select MotifCell Line:   Lab:   Protocol:   Treatment:   Antibody:  

Cell Line:HeLa-S3   Lab:Stanford   Protocol:IgG-rab   Treatment:None   Antibody:IRF3   

Binding of Other Transcription Factors or Histone Marks at the IRF3 Peaks

Select HeatmapLab:   Cellline:  

  • Comparison with the IRF3 peaks in GM12878 cells generated by SYDH

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