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E2F transcription factor 6
PDBsearch E2F6
Gene CardE2F6
UCSCBrowser view
Protein FamilyWinged helix DNA-binding domain
TypePol II TF
Ensembl Exp.Human


E2F6 is a member of the E2F family of transcription factors which play a crucial role in controlling the cell cycle, action of tumor suppressor proteins, and are a target of the transforming proteins of small DNA tumor viruses. E2F proteins contain several evolutionally conserved domains found in most members of the family, including a DNA binding domain, a dimerization domain (which determines interaction with the differentiation-regulated transcription factor proteins (DP)), a transactivation domain enriched in acidic residues, and a tumor suppressor protein association domain embedded within the transactivation domain. E2F6 lacks transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. It is a component of a multimeric protein complex that contains a histone methyltransferase and the transcription factors MGA and MAX. It regulates select E2F-dependent genes whose products are required for cell cycle entry but not progression. E2F6 overexpression delays the exit of cells from S-phase.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

HeLa-S3 1
K562 1 1

Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.

Average Profiles of Nucleosomes around the Summit of ChIP-seq Peaks

Average nucleosome occupancy profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal to an annotated transcript (red lines) and for peaks that are distal to all annotated transcripts (blue lines), as defined in the previous section. Nucleosome positioning data in GM12878 and K562 were generated by the Stanford team, with micrococcal nuclease digestion of chromatin followed by deep sequencing of mononucleosomal DNA.

Motifs Enriched in the Top 500 ChIP-seq Peaks

The sequences of the top 500 TF ChIP-seq peaks were used to identify enriched motifs de novo, using the MEME-ChIP suite of tools. Five motifs are reported (M1 to M5), with motif name, sequence logo, and number of peaks out of the top 500 peaks that contain a site for the motif. The motifs are then used to scan the entire set of ChIP-seq peaks and the two flanking (control) regions using the FIMO tool, and two quantities are reported for bins of peaks sorted by their ChIP-seq q-values: percentage of the peaks that contain a site for the motif, and the distribution of the distances of the motif site to the summit of the peak.

Select MotifCell Line:   Lab:   Protocol:   Treatment:   Antibody:  

Cell Line:HeLa-S3   Lab:USC   Protocol:std   Treatment:None   Antibody:E2F6   

Binding of Other Transcription Factors or Histone Marks at the E2F6 Peaks

Select HeatmapLab:   Cellline:  

  • Comparison with the E2F6 peaks in HELAS3 cells generated by SYDH

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