SIRT6

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sirtuin 6
SIRT6

PDB ID : 3K35
PDB3K35 3PKJ 3PKI
HGNCSIRT6
Gene CardSIRT6
Entrez51548
RefSeqNM_001193285
UniProtQ8N6T7
UCSCBrowser view
WikipediaSIRT6
Protein FamilyDHS-like NAD/FAD-binding domain
Typehistone deacetylation complexes
Ensembl Exp.Human

Function

SIRT6 (mono-ADP-ribosyltransferase sirtuin-6) is a member of the sirtuin family of proteins, homologs to the yeast SIR2 (silent information regulator 2) protein. Sirtuins regulate epigenetic gene silencing and suppress recombination of rDNA. SIRT6 is an NAD-dependent protein deacetylase. It modulates acetylation of histone H3 in telomeric chromatin during S phase. It has deacetylase activity towards H3K56, and deacetylates H3K9 at NF-kB target promoters, possibly down-regulating the expression of a subset of NF-kB target genes, as deacetylation of nucleosomes interferes with RELA binding to target DNA. SIRT6 plays a role in normal telomere maintenance, genomic stability, normal IGF1 serum levels, glucose homeostasis, cellular senescence, and apoptosis.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

+/-
Harvard
K562 1


Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.

Average Profiles of Nucleosomes around the Summit of ChIP-seq Peaks

Average nucleosome occupancy profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal to an annotated transcript (red lines) and for peaks that are distal to all annotated transcripts (blue lines), as defined in the previous section. Nucleosome positioning data in GM12878 and K562 were generated by the Stanford team, with micrococcal nuclease digestion of chromatin followed by deep sequencing of mononucleosomal DNA.

Motifs Enriched in the Top 500 ChIP-seq Peaks

The sequences of the top 500 TF ChIP-seq peaks were used to identify enriched motifs de novo, using the MEME-ChIP suite of tools. Five motifs are reported (M1 to M5), with motif name, sequence logo, and number of peaks out of the top 500 peaks that contain a site for the motif. The motifs are then used to scan the entire set of ChIP-seq peaks and the two flanking (control) regions using the FIMO tool, and two quantities are reported for bins of peaks sorted by their ChIP-seq q-values: percentage of the peaks that contain a site for the motif, and the distribution of the distances of the motif site to the summit of the peak.

Select MotifCell Line:   Lab:   Protocol:   Treatment:   Antibody:  

Cell Line:K562   Lab:Harvard   Protocol:std   Treatment:None   Antibody:SIRT6   

Binding of Other Transcription Factors or Histone Marks at the SIRT6 Peaks

Select HeatmapLab:   Cellline:  

  • Comparison with the SIRT6 peaks in K562 cells generated by SYDH

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