POL2

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polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
POL2

PDB ID : 2GHT
PDB2GHT 2GHQ
HGNC(alias)POLR2A (RPB1,POLR2,POLRA)
Gene CardPOLR2A
Entrez5430
RefSeqNM_000937
UniProtP24928
UCSCBrowser view
WikipediaPOLR2A
Protein Familybeta and beta-prime subunits of DNA dependent RNA-polymerase
TypePol II subunit
Ensembl Exp.Human

Function

POLR2A (also known as RPB1) is the largest subunit of RNA polymerase II. It contains a C-terminal domain (CTD) composed of heptapeptide repeats essential for polymerase activity. The phosphorylation status of the CTD influences whether or not elongation occurs. The CTD also serves as a platform for the assembly of a number of factors that regulate transcription. POLR2A is a component of the DNA binding domain of RNA polymerase II, and is part of the core clamp element that acts as a ratchet to open and close the cleft through which the DNA template enters and exits.

ENCODE ChIP-seq Datasets

Indicated in the matrix are the numbers of datasets specified by lab and cell line; when the number is greater than 1, multiple ChIP-seq experiments have been performed, some upon treatments. Click the numbers to download the data files on ChIP-seq peaks, alignments, etc.

+/-
Broad
Harvard
HudsonAlpha
Stanford
USC
Yale
A549 2
ECC-1 1
GM10847 1
GM12878 2 2 1
GM12891 2 1
GM12892 2 1
GM15510 1
GM18505 1
GM18526 1
GM18951 1
GM19099 1
GM19193 1
H1-hESC 2
HCT-116 1 1
HEK293 1
HeLa-S3 1 1 1 1
HepG2 2 3 1
HUVEC 1 1
K562 1 2 2 4
MCF10A-Er-Src 2
NB4 1
NHEK 1
PANC-1 1
PBDE 1
PFSK-1 1
Raji 1
SK-N-MC 1
U87 1


Average Profiles of Modified Histones around the Summit of ChIP-seq Peaks

Average histone modification profiles are shown for the [-2 kb, +2 kb] window around the summits of TF ChIP-seq peaks, separately for peaks that are proximal ([-1kb, +1kb]) to an annotated transcript (dashed lines) start sites and for peaks that are distal (more than 1kb) to all annotated transcripts (solid lines) start sites. Proximal profiles are arranged such that the transcriptional direction of the nearest transcript is toward the right. Histone modification data were generated by the Broad team, using antibodies to pull down modified histones followed by deep sequencing of the genomic DNA associated with the modified histones. Only histone modification data from the same cell line as the TF ChIP-seq data are shown.

Mouse over a curve to reveal its identity. Mouse over a histone modification in the legend to show its curves and gray out other histone modifications in the figures. Click a histone modification in the legend to toggle on/off its curve in all figures. Click the “Proximal” or “Distal” button in the legend to show only the average histone modification profiles anchored around ChIP-seq peaks that are proximal or distal to annotated transcripts.